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2025 Vol.52, Issue 4 Preview Page

Food & Chemistry

Identifying an accurate and efficient approach to soil organic matter removal for quantifying microplastics in agricultural soils
Yun-Gu Kang1†
,
Jiwon Choi1†
,
Jun-Yeong Lee1
,
Ji-Hoon Kim1
,
Jun-Ho Kim1
,
Taek-Keun Oh1*
1Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
*Corresponding Author
†These authors equally contributed to this study as first author.
1 December 2025. pp. 417-425
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Abstract

For efficient microplastic (MP) separation, there is a need to remove the soil organic matter (SOM), which interferes with MP extraction. However, the efficient SOM removal strategy from agricultural soils remain under debate. Therefore, this study investigated the SOM decomposition efficiency and MP recovery yield under varying environmental temperatures (i.e., 0, 20, and 40℃) conditions using five different removal solutions in three types of agricultural soil (i.e., upland, orchard, and greenhouse soils). Considering the thermal sensitivity and characteristics of MP samples, the study aimed to identify pre-treatment conditions that effectively decompose SOM content without significantly compromising MP recovery yield. Soil samples containing 1% (w/w) polystyrene (PS) particles were subjected to distilled water, acid-digestion (HNO3), alkaline-digestion (KOH), and oxidative (H2O2 and Fenton [H2O2 + FeSO4]) treatments, followed by density-separation using NaCl solution (1.21 g·cm-3), and the PS recovery rate was quantified after oven-drying. The SOM decomposition accelerated with increasing environmental temperature, and the its highest efficiency, which reduced SOM content by 44, 36, and 29%, were observed in the Fenton reagent-applied upland, orchard, and greenhouse soils, respectively. The MP recovery rates were positively correlated with SOM decomposition efficiency, and the oxidation reaction consistently yielded higher MP recovery yield than other approaches. However, the H2O2 treatment led to variable MP losses, particularly in low-organic soils, suggesting possible MP degradation during excessive oxidation. Hence, treating agricultural soil with the Fenton reagent at 40℃ condition was found to be the most effective strategy for decomposing SOM content while maintaining MP integrity.

Keywords
density separation
microplastic
organic matter
percentage yield
polystyrene
References
1

Chamas A, Moon H, Zheng J, Qiu Y, Tabassum T, Jang JH, Abu-Omar M, Scott SL, Suh S. 2020. Degradation rates of plastics in the environment. ACS Sustainable Chemistry & Engineering 8:3494-3511.

10.1021/acssuschemeng.9b06635
2

Do Sul JAI, Costa MF. 2014. The present and future of microplastic pollution in the marine environment. Environmental Pollution 185:352-364.

10.1016/j.envpol.2013.10.036
3

Dokl M, Copot A, Krajnc D, Fan YV, Vujanovic A, Aviso KB, Tan RR, Kravanja Z, Cucek L. 2024. Global projections of plastic use, end-of-life fate and potential changes in consumption, reduction, recycling and replacement with bioplastics to 2050. Sustainable Production and Consumption 51:498-518.

10.1016/j.spc.2024.09.025
4

Huh JH, Seo MK, Kim HY, Kim IJ, Park SJ. 2012. Influence of KOH activation on electrochemical performance of coal tar pitch-based activated carbons for supercapacitor. Polymer (Korea) 36:756-760. [in Korean]

10.7317/pk.2012.36.6.756
5

Hurley RR, Lusher AL, Olsen M, Nizzetto L. 2018. Validation of a method for extracting microplastics from complex, organic-rich, environmental matrices. Environmental Science & Technology 52:7409-7417.

10.1021/acs.est.8b01517
6

IBM. 2019. IBM SPSS Statistics for Windows. Ver. 26. Armonk, NY: IBM Corp.

7

Ivanic FM, Guggenberger G, Woche SK, Bachmann J, Hoppe M, Carstens FJ. 2023. Soil organic matter facilitates the transport of microplastic by reducing surface hydrophobicity. Colloids and Surfaces A: Physicochemical and Engineering Aspects 676:132255.

10.1016/j.colsurfa.2023.132255
8

Jeon YJ, Kang YG, Eun JA, Oh TK. 2024. Yield, functional properties and nutritional compositions of leafy vegetables with dehydrated food waste and spent coffee grounds. Applied Biological Chemistry 67:22.

10.1186/s13765-024-00863-0
9

Kang YG, Choi J, Lee JY, Kim JH, Kim JH, Park DG, Oh TK, Yun YU. 2024. Response of biochar amendment for substituting organic fertilizer ingredients in kale-grown soils. Korean Journal of Agricultural Science 51:819-827. [in Korean]

10.7744/kjoas.510430
10

Kang YG, Lee JH, Lee JY, Kim JH, Oh TK. 2023. Effects of biochar-based fertilizer on ammonia volatilization under controlled conditions. Korean Journal of Agricultural Science 50:479-488. [in Korean]

10.7744/kjoas.500313
11

Kim JH, Kang YG, Lee JY, Kim JH, Choi JW, Oh TK. 2024. Influences of silicate fertilizers containing different rates of iron slag on CH4 emission and rice (Oryza sativa L.) growth. Korean Journal of Agricultural Science 51:227-238. [in Korean]

10.7744/kjoas.510213
12

Koelmans AA, Redondo-Hasselerharm PE, Nor NHM, de Ruijter VN, Mintenig SM, Kooi M. 2022. Risk assessment of microplastic particles. Nature Reviews Materials 7:138-152.

10.1038/s41578-021-00411-y
13

Lavoy M, Crossman J. 2021. A novel method for organic matter removal from samples containing microplastics. Environmental Pollution 286:117357.

10.1016/j.envpol.2021.117357
14

Lee JY, Park DG, Kang YG, Kim JH, Kim JH, Choi JW, Yun YU, Oh TK. 2024. Effect of biochar derived from rice husk and chicken manure on lettuce growth and soil chemical properties. Korean Journal of Agricultural Science 51:271-281. [in Korean]

10.7744/kjoas.510303
15

Lee Y, Choi S, Lee J, An H, Lee CH, Yi PH, Jeong ST, Kim SY. 2021. Characteristics of distribution and decomposition of organic matter in soils cultivated with various fruits and vegetables in plastic film house fields. Korean Journal of Soil Science and Fertilizer 54:401-412. [in Korean]

10.7745/KJSSF.2021.54.4.401
16

Masura J, Baker J, Foster G, Arthur C. 2015. Laboratory Methods for the Analysis of Microplastics in the Marine Environment: Recommendations for Quantifying Synthetic Particles in Waters and Sediments. NOAA Technical Memorandum NOS-OR&R-48.

17

Nabi I, Bacha A, Zhang L. 2022. A review on microplastics separation techniques from environmental media. Journal of Cleaner Production 337:130458.

10.1016/j.jclepro.2022.130458
18

Oh S, Park J, Jung S, Jeong J, Park K. 2021. Study on the Fenton reaction condition for evaluation of chemical durability of PEMFC membrane. Korean Chemical Engineering Research 59:49-53. [in Korean]

10.9713/KCER.2021.59.1.49
19

Porterfield KK, Hobson SA, Neher DA, Niles MT, Roy ED. 2023. Microplastics in composts, digestates, and food wastes: A review. Journal of Environmental Quality 52:225-240.

10.1002/jeq2.20450
20

Prata JC, da Costa JP, Girão AV, Lopes I, Duarte AC, Rocha-Santos T. 2019. Identifying a quick and efficient method of removing organic matter without damaging microplastic samples. Science of the Total Environment 646:131-139.

10.1016/j.scitotenv.2019.05.456
21

Shin JH, Kang YG, Lee JY, Kim JH, Oh TK. 2024. Effect of poultry manure biochar on soil nutrition and carbon improvement in Saemangeum reclaimed soils. Korean Journal of Agricultural Science 51:643-654. [in Korean]

10.7744/kjoas.510418
22

Shin Y. 2025. Analysis of soil carbon dioxide variability characteristics in forest, agriculture and meadow. Journal of the Korean Society of Agricultural Engineers 67:25-32. [in Korean]

10.5389/KSAE.2025.67.2.025
Information
  • Publisher :Institute of Agricultural Science, Chungnam National University
  • Publisher(Ko) :충남대학교 농업과학연구소
  • Journal Title :Korean Journal of Agricultural Science
  • Journal Title(Ko) :농업과학연구
  • Volume : 52
  • No :4
  • Pages :417-425
  • Received Date : 2025-05-19
  • Revised Date : 2025-06-12
  • Accepted Date : 2025-06-13
  • DOI :https://doi.org/10.7744/kjoas.520403
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