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2026 Vol.53, Issue 1 Preview Page

Animal

Effects of Isulsongi mushroom (Lentinula edodes GNA01) supplementation level and feeding period on serum lipid profiles in dogs
Eun-Gyeom Jung1
,
Soon Hwangbo2*
1Department of Pet Beauty and Behavior Education, Tongmyong University, Busan 48520, Korea
2Department of Companion Animal Industry, Daegu University, Gyeongsan 38453, Korea
*Corresponding Author
1 March 2026. pp. 75-84
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Abstract

This study examined how the feeding level and duration of Isulsongi mushroom (Lentinula edodes GNA01) affected blood lipid profiles in dogs. Eighteen Maltese dogs were split into three groups: control (no supplement), 0.01% (100 mg·kg-1), and 0.02% (200 mg·kg-1). The mushroom powder was given by mouth for nine weeks. Blood was collected at 0, 3, 6, and 9 weeks to measure total cholesterol (TC), triglycerides (TGs), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C). From these data, atherogenic index (AI) and cardiac risk factor (CRF) were calculated. Data were analyzed by feeding level, feeding period, and their interaction. Dogs supplemented with the mushroom showed lower TC, TGs, and LDL-C and higher HDL-C than the control group. The 0.02% group showed the lowest TC and LDL-C and the highest HDL-C (p < 0.05). AI and CRF decreased as the supplementation level and time increased. These results indicate that Isulsongi mushroom helps regulate lipid levels and could be used as a functional ingredient for dogs.

Keywords
dogs
HDL-cholesterol
LDL-cholesterol
Lentinula edodes GNA01
serum lipids
References
1

Alam N, Yoon KN, Lee TS, Lee UY. 2011. Hypolipidemic activities of dietary Pleurotus ostreatus in hypercholesterolemic rats. Mycobiology 39:45-51.

10.4489/MYCO.2011.39.1.04522783072PMC3385090
2

Cheung PCK. 1996. The hypocholesterolemic effect of extracellular polysaccharide from the submerged fermentation of mushroom. Nutrition Research 16:1953-1957.

10.1016/S0271-5317(96)00218-7
3

Im KH, Baek SA, Choi JH, Lee TS. 2021. Anti-hyperlipidemic and anti-obesity effects of Sparassis latifolia fruiting bodies in high-fat and cholesterol-diet-induced hyperlipidemic rats. Journal of Mushrooms 19:23-32. [in Korean]

10.14480/JM.2021.19.1.23
4

Jang HL, Lee JH, Hwang MJ, Choi YM, Kim HR, Hwang JB, Nam JS. 2015. Comparison of physicochemical properties and antioxidant activities between Lentinula edodes and new cultivar Lentinula edodes GNA01. Journal of the Korean Society of Food Science and Nutrition 44:1484-1491. [in Korean]

10.3746/jkfn.2015.44.10.1484
5

Jang HL, Park SY, Nam JS. 2017. Effect of extraction solvent on the antioxidant activity of Lentinula edodes GNA01 extract. The Korean Journal of Food and Nutrition 30:51-58. [in Korean]

10.9799/ksfan.2017.30.1.051
6

Jang HS, Yoon KN. 2017. The antihyperlipidemic effect of lion’s mane mushroom (Hericium erinaceus) in hyperlipidemic rats induced by high fat and cholesterol diet. Korean Journal of Clinical Laboratory Science 49:263-270. [in Korean]

10.15324/kjcls.2017.49.3.263
7

Jin GH, Lee TS. 2018. Anti-hyperlipidemic effect of Agaricus brasiliensis fruiting bodies in rats fed with high cholesterol diet. Journal of Mushrooms 16:118-124. [in Korean]

10.14480/JM.2018.16.2.118
8

Jung EG, Hwangbo S. 2025. Effects of Isulsongi mushroom (Lentinula edodes GNA01) supplementation on age-dependent blood lipid characteristics in dogs. Korean Journal of Agricultural Science 52:653-661.

10.7744/kjoas.520420
9

Kim EJ, Choe CY, Yoo JG, So KM, Jung YH, Cho AR, Kim SH, Oh SI, Do YJ. 2018. Comparison of hematological and serum biochemical parameters among small breed dogs. Korean Journal of Veterinary Service 41:1-8. [in Korean]

10.7853/KJVS.2018.41.1.1
10

Kim GY, Jung HW, Kim EJ. 2013. Anti-hyperlipidemic effect of shiitake mushroom extract in hyperlipidemic rats induced by Poloxamer-407. Journal of Physiology & Pathology in Korean Medicine 27:409-415. [in Korean]

11

Kim JH. 2019. Antihyperlipidemic effects of edible mushrooms on the hyperlipidemic rats fed high fat and cholesterol diets. Ph.D. dissertation, Incheon National Univ., Incheon, Korea. [in Korean]

12

Kim NY. 2010. Comparative studies on haematology and aggressive behavior of domestic house dogs and stray dogs. Master’s thesis, Konkuk Univ., Seoul, Korea. [in Korean]

13

Kusaba A, Okada Y, Ueno H, Yamamoto I, Mori Y, Tanaka N, Arai T, Kawasumi K. 2021. Effects of supplementation with Shiitake powder, Lentinula edodes, on anti-oxidative activities and energy/lipid metabolism in healthy dogs. Research Square [Preprint]. doi:10.21203/rs.3.rs-1199968/v1

10.21203/rs.3.rs-1199968/v1
14

Lee EC, Park SJ, Lee JH, Jeong HH, Joo JW, Lee SR. 2020. Starter culture selection and verification for senior dog fermented food development. Annals of Animal Resource Sciences 31:87-101. [in Korean]

10.12718/AARS.2020.31.3.87
15

Lee MY, Cho HW, Chun JL, So KM, Lee SY, Kim KH. 2025. Evaluation of age- and body weight-related variations in canine blood biochemical parameters. Journal of Korea Academia-Industrial Cooperation Society 26:58-67. [in Korean]

10.5762/KAIS.2025.26.4.58
16

Mori K, Kobayashi C, Tomita T, Inatomi S, Ikeda M. 2008. Antiatherosclerotic effect of the edible mushrooms Pleurotus eryngii (Eringi), Grifola frondosa (Maitake), and Hypsizygus marmoreus (Bunashimeji) in apolipoprotein E-deficient mice. Nutrition Research 28:335-342.

10.1016/j.nutres.2008.03.010
17

Na HM, Choi JW, Park JS, Lee YE, Bae SY, Park SD, Kim ES, Kim YH. 2014. The health status of stray dogs and cats adopted to public animal shelter in Gwangju area, Korea. Korean Journal of Veterinary Service 37:281-290. [in Korean]

10.7853/kjvs.2014.37.4.281
18

Qu L, Xin H, Zheng G, Su Y, Ling C. 2012. Hepatoprotective activity of the total saponins from Actinidia valvata Dunn root against carbon tetrachloride-induced liver damage in mice. Evidence-Based Complementary and Alternative Medicine 2012:216061.

10.1155/2012/21606123243434PMC3518303
19

SAS. 2023. SAS User’s Guide: Statistics. Ver. 9.4. Cary, NC: SAS Institute, Inc.

20

Song WY, Sung BH, Kang SK, Choi JH. 2010. Effect of water extracts from Phellinus linteus on lipid composition and antioxidative system in rats fed high fat high cholesterol diet. Journal of the Korean Society of Food Science and Nutrition 39:71-77. [in Korean]

10.3746/jkfn.2010.39.1.071
21

Usui S, Yasuda H, Koketsu Y. 2015. Lipoprotein cholesterol and triglyceride concentrations associated with dog body condition score; effect of recommended fasting duration on sample concentrations in Japanese private clinics. Journal of Veterinary Medical Science 77:1063-1069.

10.1292/jvms.15-003225866404PMC4591146
22

Wasser SP. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Applied Microbiology and Biotechnology 60:258-274.

10.1007/s00253-002-1076-7
23

Yoon KN, Alam N, Lee JS, Cho HJ, Kim HY, Shim MJ, Lee MW, Lee TS. 2011a. Antihyperlipidemic effect of dietary Lentinus edodes on plasma, feces and hepatic tissues in hypercholesterolemic rats. Mycobiology 39:96-102.

10.4489/MYCO.2011.39.2.09622783084PMC3385098
24

Yoon KN, Alam N, Shim MJ, Lee TS. 2011b. Hypolipidemic and antiatherogenesis effect of culinary-medicinal pink oyster mushroom, Pleurotus salmoneostramineus L. Vass. (higher basidiomycetes), in hypercholesterolemic rats. International Journal of Medicinal Mushrooms 14:27-36.

10.1615/IntJMedMushr.v14.i1.30
25

Youn AR, Lee YJ. 2021. Basic research on chemical components, mineral content and antioxidant activities of dewdrop pine mushroom (Lentinula edodes GNA01). Culinary Science & Hospitality Research 27:178-184. [in Korean]

10.20878/cshr.2021.27.11.016
26

Zhang M, Cui SW, Cheung PCK, Wang Q. 2007. Antitumor polysaccharides from mushrooms: A review on their isolation process, structural characteristics and antitumor activity. Trends in Food Science & Technology 18:4-19.

10.1016/j.tifs.2006.07.013
27

Zhu F, Zhang Q, Feng J, Zhang X, Li T, Liu S, Chen Y, Li X, Wu Q, Xue Y, et al. 2023. β-Glucan produced by Lentinus edodes suppresses breast cancer progression via the inhibition of macrophage M2 polarization by integrating autophagy and inflammatory signals. Immunity, Inflammation and Disease 11:e876.

10.1002/iid3.87637249285PMC10214582
Information
  • Publisher :Institute of Agricultural Science, Chungnam National University
  • Publisher(Ko) :충남대학교 농업과학연구소
  • Journal Title :Korean Journal of Agricultural Science
  • Journal Title(Ko) :농업과학연구
  • Volume : 53
  • No :1
  • Pages :75-84
  • Received Date : 2026-01-15
  • Revised Date : 2026-02-24
  • Accepted Date : 2026-02-25
  • DOI :https://doi.org/10.7744/kjoas.530107
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