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2025 Vol.52, Issue 2 Preview Page

Plant & Forest

Acute toxicity evaluation to Cyprinus carpio of herbicide resistance transgenic chrysanthemum
Ye Jin Jang1
,
Young Jin Yeo1
,
Eun Jung Suh1
,
Jong-Chan Park1
,
Seong-Kon Lee1
,
Doh-Won Yun1
,
Ancheol Chang1
,
Seok-Ki Min2
,
Sung-Dug Oh1*
1Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea
2Korea Testing & Research Institute, Hwasun 58151, Korea
*Corresponding Author
1 June 2025. pp. 201-211
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Abstract

Herbicide-resistant transgenic chrysanthemum was generated by inserting the phosphinothricin acetyltransferase (pat) gene into the plant genome. The acute toxicity effects of herbicide-resistant transgenic chrysanthemum (genetically modified, GM) and non-GM chrysanthemum on Cyprinus carpio were investigated at various concentrations (0, 156, 131, 625, 1,250, and 2,500 mg·L-1). Through polymerase chain reaction (PCR) and immunostrip analysis, it was confirmed that the GM chrysanthemum exhibited resistance to glufosinate herbicides due to the introduction of the pat gene. To evaluate the environmental risks of GM chrysanthemum, feeding experiments were conducted on C. carpio. The fish were fed with ground GM chrysanthemum or non-GM chrysanthemum. The 96 h-LC50 (lethal concentration 50) values were 752.0 mg·L-1 (95% confidence limits: 675.8 - 836.9 mg·L-1) and 788.5 mg·L-1 (95% confidence limits: 686.4 - 905.8 mg·L-1) for GM and non-GM chrysanthemum, respectively. Additionally, no significant differences in body weight and length were observed in C. carpio fed with GM and non-GM chrysanthemum. These results indicate that there were no significant differences in toxicity or physiological effects on the C. carpio between GM chrysanthemum and its non-GM counterpart.

Keywords
Cyprinus carpio
genetically modified (GM) chrysanthemum
risk assessment
References
1

Agbioinvestor. 2024. Global GM crop area review. Accessed in http://gm.agbioinvestor.com on 5 June 2024.

2

Carstens K, Anderson J, Bachman P, Schrijver AD, Dively G, Federici B, Hamer M, Gielkens M, Jensen P, Lamp W, et al. 2012. Genetically modified crops and aquatic ecosystems: Considerations for environmental risk assessment and non-target organism testing. Transgenic Research 21:813-842.

10.1007/s11248-011-9569-822120952PMC3394238
3

Hilbeck A, Bundschuh R, Bundschuh M, Hofmann F, Oehen B, Otto M, Schulz R, Trtikovay M. 2017. Procedure to select test organisms for environmental risk assessment of genetically modified crops in aquatic systems. Integrated Environmental Assessment and Management 13:974-979.

10.1002/ieam.196528755496
4

IBM. 2020. IBM SPSS Statistics for Windows. Ver. 23.0. Armonk, NY: IBM Corp.

5

Jeong JH, Eun JS, Joo CH, Jo AH, Hong SM, Kim JH. 2022. Effects on lethal concentration 50%, hematological parameters and plasma components of mirror carp, Cyprinus carpio nudus exposed to waterborne ammonia. Journal of Fish pathology 35:65-75. [in Korean]

10.7847/jfp.2022.35.1.065
6

KBCH (Korea Biosafety Clearing House). 2024. Major Statistics on Genetically Modified Organisms in 2023. [in Korean]

7

Kim DC, Lee YH, Kim SL, Kim NH, Park KY, Lee JS. 2023. Breeding of the garden chrysanthemum 'Burning Ball', a semi-upright type with a red color. Korean Journal of Breeding Science 55:48-53. [in Korean]

10.9787/KJBS.2023.55.1.48
8

Lee SY, Cheon KS, Kim SY, Shin JY, Lee YA, Ramya MR, Kang YI, Park PH, Park PM, An HR. 2017. Functional expression of flavonoid 3′,5′-hydroxylase (F3′5′H) gene isolated from Clematis patens in petunia. Flower Research Journal 25:246-252. [in Korean]

10.11623/frj.2017.25.4.09
9

Li Y, Peng Y, Hallerman EM, Wu K. 2014. Biosafety management and commercial use of genetically modified crops in China. Plant Cell Reports 33:565-573.

10.1007/s00299-014-1567-x24493253
10

Lin LZ, Harnly JM. 2010. Identification of the phenolic components of chrysanthemum flower (Chrysanthemum morifolium Ramat). Food Chemistry 120:319-326.

10.1016/j.foodchem.2009.09.083
11

Miki B, McHugh S. 2004. Selectable marker genes in transgenic plants: Applications, alternatives and biosafety. Journal of Biotechnology 107:193-232.

10.1016/j.jbiotec.2003.10.01114736458
12

Oh MJ, Kim JH, Ahn MS, Liu JR, Kim SW. 2010. Recent advances in development of commercial rose by molecular breeding. Journal of Plant Biotechnology 37:414-424. [in Korean]

10.5010/JPB.2010.37.4.414
13

Oh SD, Jang YJ, Park SY, Suh SJ, Lee BK. 2020. Characterization of hybrid soybean seeds between β-carotene enhanced transgenic soybean and wild soybean. Weed & Turfgrass Science 9:129-139. [in Korean]

10.5660/WTS.2020.9.2.129
14

Oh SD, Kim JS, Lee DY, Ryu TH, Suh SJ. 2015. Risk assessment and evaluation of drought-tolerant transgenic rice: Responses of Misgurnus anguillicaudatus and Cyprinus carpio fed on drought-tolerant transgenic rice variety. Journal of the Korean Society of International Agriculture 27:497-504. [in Korean]

10.12719/KSIA.2015.27.4.497
15

Oh SD, Lee DY, Sohn SI, Lee KJ, Ryu TH, Lee JY, Park BS, Kweon SJ, Suh SC, Park JS. 2011. Risk assessment and evaluation of Bt-transgenic rice: Responses of Misgurnus anguillicaudatus and Cyprinus carpio fed on Bt-transgenic rice variety. Journal of the Korean Society of International Agriculture 23:570-577. [in Korean]

16

Oh SD, Lee KJ, Park SY, Lee DY, Sohn SI, Kim MY, Ryu TH. 2013. Responses of Misgurnus anguillicaudatus and Cyprinus carpio fed on disease resistant (OsCK1) rice variety. Korean Journal of Environmental Agriculture 32:231-239. [in Korean]

10.5338/KJEA.2013.32.3.231
17

Oh SD, Won SY, Lee KJ, Sohn SI, Lee SM, Park SK, Ryu TH. 2014. Molecular biological characteristics and biosafety assessment for drought-tolerant transgenic rice (Agb0103). Korean Journal of Breeding Science 46:389-399. [in Korean]

10.9787/KJBS.2014.46.4.389
18

Su J, Jiang J, Zhang F, Liu Y, Ding L, Chen S, Chen F. 2019. Current achievements and future prospects in the genetic breeding of chrysanthemum: A review. Horticulture Research 6:109.

10.1038/s41438-019-0193-831666962PMC6804895
19

Suh EJ, Hong JK, Lee YH, Kim DC. 2020. Overexpression of the Brassica rapa SRS7 gene in pot-type chrysanthemum [Chrysanthemum morifolium Ramat] reduces plant height. Scientia Horticulturae 273:109634.

10.1016/j.scienta.2020.109634
20

Suharman I, Satoh S, Haga Y, Takeuchi T, Hirono I, Aoki T. 2010. Suitability of genetically modified soybean meal in a dietary ingredient for common carp Cyprinus carpio. Fisheries Science 76:111-117.

10.1007/s12562-009-0183-0
21

Teixeira da Silva JA, Shinoyama H, Aida R, Matsushita Y, Raj SK, Chen F. 2013. Chrysanthemum Biotechnology: Quo vadis? Critical Reviews in Plant Sciences 32:21-52.

10.1080/07352689.2012.696461
22

Tidepool Scientific. 2016. CETIS. Ver. 1.9.3.0. McKinleyville, CA: Tidepool Scientific, LLC.

Information
  • Publisher :Institute of Agricultural Science, Chungnam National University
  • Publisher(Ko) :충남대학교 농업과학연구소
  • Journal Title :Korean Journal of Agricultural Science
  • Journal Title(Ko) :농업과학연구
  • Volume : 52
  • No :2
  • Pages :201-211
  • Received Date : 2025-03-07
  • Revised Date : 2025-05-26
  • Accepted Date : 2025-05-26
  • DOI :https://doi.org/10.7744/kjoas.520212
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