Korean Journal of Agricultural Science (Korean J. Agric. Sci.; KJOAS)
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Sevilleno SS, Cabahug-Braza RA, An HR, Lim KB, Hwang YJ. The role of cytogenetic tools in orchid breeding. Korean Journal of Agricultural Science 50:235-248.
Korean Journal of Agricultural Science (Korean J. Agric. Sci.) 2023 June, Volume 50, Issue 2, pages 50:235-248. https://doi.org/10.7744/kjoas.20230015
Received on 18 January 2023, Revised on 30 March 2023, Accepted on 20 April 2023, Published on 30 June 2023.
Samantha Sevilleno Sevilleno1, Raisa Aone Cabahug-Braza2, Hye Ryun An3, Ki‑Byung Lim4, Yoon-Jung Hwang1,2,*
1Department of Convergence Science, Sahmyook University, Seoul 01795, Korea
2Plant Genetics and Breeding Institute, Sahmyook University, Seoul 01795, Korea
3Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Korea
4Department of Horticulture Science, Kyungpook National University, Daegu 41566, Korea
*Corresponding author: hyj@syu.ac.kr
Orchidaceae species account for one-tenth of all angiosperms including more than 30,000 species having significant ecological, evolutionary, and economic importance. Despite Orchidaceae being one of the largest families among flowering plants, crucial cytogenetic information for studying species diversification, inferring phylogenetic relationships, and designing efficient breeding strategies is lacking, except for 10% or less of orchid species cases involving mostly chromosome number or karyotype analysis. Also, only approximately 1.5% of the identified orchid species from less than a hundred genera have genome size data that provide crucial information for breeders and molecular geneticists. Various molecular cytogenetic techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), have been developed for determining ploidy levels, analyzing karyotypes, and evaluating hybridity, in several ornamental crops including orchids. The estimation of genome size and the determination of nuclear DNA content using flow cytometry have also been employed in some Orchidaceae subfamilies. These different techniques have played an important role in supplementing beneficial knowledge for effective plant breeding programs and other related plant research. This review focused on orchid breeding summarizes the status of current cytogenetic tools in terms of background, advancements, different techniques, significant findings, and research challenges. Principal roles and applications of cytogenetics in orchid breeding as well as different ploidy level determination methods crucial for breeding are also discussed.
breeding, chromosome, cytogenetics, FISH (fluorescence in situ hybridization), orchids
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Samantha S. Sevillen, https://orcid.org/0000-0001-9906-4992
Raisa Aone M. Cabahug, https://orcid.org/0000-0003-0863-4721
Hye Ryun An, https://orcid.org/0000-0002-3606-9195
Ki‑Byung Lim, https://orcid.org/0000-0003-2342-4052
Yoon-Jung Hwang, https://orcid.org/0000-0002-5984-8968
No potential conflict of interest relevant to this article was reported.
This work was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01502405), Rural Development Administration, Republic of Korea.