Korean Journal of Agricultural Science (Korean J. Agric. Sci.; KJOAS)
Indexed in KCI (Korea Citation Index), Open Access, Peer Reviewed.
pISSN 2466-2402
eISSN 2466-2410

Metagenome analysis: organic fruit thinner has impact on microbial community of Ginkgo biloba rhizosphere



Lee SH, Seo JB, Park JY, Choi AH, Kim DS, Kim DY, Jeon SH, Lim HM, Park WJ, Park SY, Park SJ, Kim SH, Jo JD, Kang JW. Metagenome analysis: organic fruit thinner has impact on microbial community of Ginkgo biloba rhizosphere. Korean Journal of Agricultural Science 50:181-189.

Korean Journal of Agricultural Science (Korean J. Agric. Sci.) 2023 March, Volume 50, Issue 1, pages 50:181-189. https://doi.org/10.7744/kjoas.20230012

Received on 01 December 2022, Revised on 01 December 2022, Accepted on 28 February 2023, Published on 30 March 2023.

Metagenome analysis: organic fruit thinner has impact on microbial community of Ginkgo biloba rhizosphere

Seok Hui Lee1, Jong-Beom Seo2, Jun Young Park1, Ah Hyeon Choi1, Dae Sol Kim1, Do Yeon Kim1, Su Hong Jeon1, Hee Min Lim1, Woo Jin Park1, Sin Young Park1, Sang Jun Park1, Seong-Ho Kim3, Jae Doo Jo4, Jun Won Kang1,*

1Department of Forestry, Kyungpook National University, Daegu 41566, Korea

2Forest Microbiology Division, National Institute of Forest Science, Suwon 16631, Korea

3Jinyangsa Corp., Jeonju 54882, Korea

4Ecofarm Corp., Daegu 41566, Korea

*Corresponding author: jwkang15@knu.ac.kr


Ginkgo biloba is a popular tree species as a roadside tree because of its high resistance to air pollution, high temperature, dryness, and beautiful foliage, but many complaints occur due to the odor and street pollution caused by the maturation of seeds and falling fruits in autumn. To solve this problem, fruit thinning organic chemicals have been injected and sprayed into the rhizosphere of Ginkgo biloba. In this study, to investigate the safety of the chemicals, changes in the microbial community in the rhizosphere were analyzed using next-generation sequencing (NGS) and Quantitative Insights Into Microbial Ecology 2 (QIIME2). The results showed that there was no significant difference in the soil chemical changes between the April and August of treated and control groups. In contrast, there was a significant difference (p < 0.05) in bacteria alpha diversity between the treated group and the untreated group in May. However, the soil quickly recovered to a level where the difference was not significant. With regard to beta diversity, there was a significant difference (p < 0.05) between the treated group and the untreated group. Therefore, it is considered that the microbiome was changed by applying organic fruit thinning organic chemicals. The results of this study suggest that soil metagenomic analysis could be used as an indicator of soil health and change.


Ginkgo biloba, metagenomic analysis, microbiome, next generation sequencing, organic fruit thinner


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Authors Information

Seok Hui Lee, https://orcid.org/0000-0002-2209-1500

Jong-Beom Seo, https://orcid.org/0000-0001-6863-5983

Jun Young Park, https://orcid.org/0000-0003-4655-3092

Ah Hyeon Choi, https://orcid.org/0000-0002-5114-5512

Dae Sol Kim, https://orcid.org/0000-0002-7137-3133

Do Yeon Kim, https://orcid.org/0000-0002-1407-5949

Su Hong Jeon, https://orcid.org/0000-0002-6668-8632

Hee Min Lim, https://orcid.org/0000-0002-9197-1560

Woo Jin Park, https://orcid.org/0000 0002 3511 2585

Sin Young Park, https://orcid.org/0000-0002-3443-3379

Sang Jun Park, Department of Forestry, Kyungpook National University, professor

Seong-Ho Kim, Jinyangsa Corp., CEO

Jae Doo Jo, Ecofarm Corp., CEO

Jun Won Kang, https://orcid.org/0000-0003-3641-4654


본 연구는 산림청(한국임업진흥원) 산림과학기술 연구개발사업(FTIS 2019149C10-2323-0301 및 FTIS 2021299D10-2121-AD02)의 지원에 의해 수행되었습니다.

Conflicts of interest

No potential conflict of interest relevant to this article was reported.