SY Lee, Kong HG, Kang IJ, Oh H, Woo HJ, Roh E. Identification of virulence-associated genes of Erwinia amylovora by transposon mutagenesis. Korean Journal of Agricultural Science 50:283-289.
Korean Journal of Agricultural Science (Korean J. Agric. Sci.) 2023 June, Volume 50, Issue 2, pages 50:283-289. https://doi.org/10.7744/kjoas.202300019
Received on 31 January 2023, Revised on 27 March 2023, Accepted on 26 April 2023, Published on 30 June 2023.
Seung Yeup Lee1, Hyun Gi Kong2, In Jeong Kang3, Hyeonseok Oh4, Hee-Jong Woo4, Eunjung Roh1,*
1Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea
2College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea
3Department of Central Area Crop Science, National Institute of Crop Science, Suwon 16613, Korea
4National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea
*Corresponding author: rosalia51@korea.kr
Erwinia amylovora, which causes fire blight disease on apple and pear trees, is one of the most important phytopathogens because of its devastating impact. Currently, the only way to effectively control fire blight disease is through the use of antibiotics such as streptomycin, kasugamycin, or oxytetracycline. However, problems with the occurrence of resistant strains due to the overuse of antibiotics are constantly being raised. It is therefore necessary to develop novel disease control methods through an advanced understanding of the pathogenesis mechanism of E. amylovora. To better understand the pathogenesis of E. amylovora, we investigated unknown virulence factors by random mutagenesis and screening. Random mutants were generated by Tn5 transposon insertion, and the pathogenicity of the mutants was assessed by inoculation of the mutants on apple fruitlets. A total of 17 avirulent mutants were found through screening of 960 random mutants. Among them, 14 mutants were already reported as non-pathogenic strains, while three mutants, TS3128_M2899 (ΔSUFU), TS3128_M2939 (ΔwcaG), and TS3128_M3747 (ΔrecB), were not reported. Further study of the association between E. amylovora pathogenicity and these 3 novel genes may provide new insight into the development of control methods for fire blight disease.
Erwinia amylovora, fire blight, transposon mutagenesis, virulence factor
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Seung Yeup Lee, https://orcid.org/0000-0002-8768-7276
Hyun Gi Kong, Department of plant medicine, Chungbuk National University, Professor
In Jeong Kang, https://orcid.org/0000-0001-6689-0024
Hyeonseok Oh, https://orcid.org/0000-0002-1673-5170
Hee-Jong Woo, https://orcid.org/0000-0003-0922-410X
Eunjung Roh, https://orcid.org/0000-0003-2999-5817
No potential conflict of interest relevant to this article was reported.
본 논문은 농촌진흥청 연구사업(화상병균의 유전체 분석 및 병원성 관련 유전자 선발, PJ01493403)의 지원에 의해 이루어진 것임.