Korean Journal of Agricultural Science (Korean J. Agric. Sci.; KJOAS)
Indexed in KCI (Korea Citation Index), Open Access, Peer Reviewed.
pISSN 2466-2402
eISSN 2466-2410

Detection of frog and aquatic insects by environmental DNA in paddy water ecology



Kim K, Kwon S, Noh A. Detection of frog and aquatic insects by environmental DNA in paddy water ecology. Korean Journal of Agricultural Science 50:299-312.

Korean Journal of Agricultural Science (Korean J. Agric. Sci.) 2023 June, Volume 50, Issue 2, pages 50:299-312. https://doi.org/10.7744/kjoas.20230021

Received on 03 February 2023, Revised on 28 February 2023, Accepted on 26 April 2023, Published on 30 June 2023.

Detection of frog and aquatic insects by environmental DNA in paddy water ecology

Keonhee Kim1,*, Sera Kwon2, Alongsaemi Noh1

1Human and Eco Care Center, Konkuk University, Seoul 05029, Korea

2Department of Eco Science, Ehwa Woman University, Seoul 03760, Korea

*Corresponding author: passbosko@gmail.com


The paddy environment is classified as a wetland and occupies a very large proportion of the freshwater environment. It is also ecologically important as a habitat and spawning ground for many aquatic insects and amphibian larvae. However, due to climate change and indiscriminate spraying of pesticides, the rice field ecosystem is continuously threatened. In order to restore ecologically damaged rice paddies in the future, information on organisms living in the rice paddy ecosystem, which can serve as a restoration standard, is needed. The eDNA metabarcoding analysis method is a very effective means of accumulating information on many organisms living in the rice field ecosystem because it can indirectly identify the existence of taxa that are no longer found in the target ecosystem due to different adult life periods or metamorphosis. In this study, genes of four species of frogs and nine species of aquatic insects were also discovered, and some taxa were directly discovered in the field. A large number of taxa have been discovered only by DNA searches, and traditional survey methods have only been able to identify very limited taxa. This eDNA-based paddy field biosearch is expected to be very useful in the investigation of biodiversity in agricultural ecosystems due to its strong analytical resolution.


Amphibia, aquatic insect, detection, eDNA, paddy environment


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Authors Information

Kim Keonhee, https://orcid.org/0000-0002-5725-1447

Kwon Sera, https://orcid.org/0000-0003-3001-6663

Noh Alongsaemi, https://orcid.org/0000-0003-2073-4573

Conflicts of interest

No potential conflict of interest relevant to this article was reported.


본 논문은 농촌진흥청 공동연구사업(과제번호: PJ015071042023)의 지원에 의해 이루어진 것임.