Korean Journal of Agricultural Science (Korean J. Agric. Sci.; KJOAS)
Indexed in KCI (Korea Citation Index), Open Access, Peer Reviewed.
pISSN 2466-2402
eISSN 2466-2410

Anti-obesity effects of two herbal extracts in C57BL/6N mice fed high-fat diet



Kim H, Lee J, Chang S, Song D, An J, Park S, Jeon K, Choi J, Cho J. 2024. Anti-obesity effects of two herbal extracts in C57BL/6N mice fed high-fat diet. Korean Journal of Agricultural Science 51:87-95.

Korean Journal of Agricultural Science (Korean J. Agric. Sci.) 2024 March, Volume 51, Issue 1, pages 87-95. https://doi.org/10.7744/kjoas.510108

Received on 04 December 2023, Revised on 22 January 2024, Accepted on 21 February 2024, Published on 1 March 2024.

Anti-obesity effects of two herbal extracts in C57BL/6N mice fed high-fat diet

Hyuck Kim1,†, Jihwan Lee2,†, Seyeon Chang1, Dongcheol Song1, Jaewoo An1, Sehyun Park1, Kyeongho Jeon1, Jungseok Choi1,*, Jinho Cho1,*

1Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea

2Department of Poultry Science, University of Georgia, Athens, GA 30602, USA

These authors equally contributed to this study as first author.

*Corresponding author: jchoi@chungbuk.ac.kr, jinhcho@cbnu.ac.kr


The objective of this study was to investigate the anti-obesity effects of adding Momordica charantia (MC) and Chrysanthemum zawadskii var. latilobum (CZ) extracts to drinking water on obesity-induced mice. A total of 84 eight-week-old C57BL/6N male mice with an initial body weight (BW) of 28.11 ± 1.39 g were used in this study. All treatments were fed a highfat diet for d 28. Mice were randomly divided into seven drinking treatments (six replicate each treatment) based on the initial BW. Treatments are as follows: control (CON), normal tap water, MC 1, CON with 1% MC aqueous extract, MC 2, CON with 2% MC aqueous extract, CZ 1, CON with 1% CZ aqueous extract, CZ 2, CON with CZ aqueous extract (2%), MCZ 1, CON with 1% MC aqueous extract and 1% CZ aqueous extract, MCZ 2, CON with 2% MC aqueous extract and 2% CZ aqueous extract. During the entire period, the CZ 1, MCZ 1, and MCZ 2 significantly decreased (p < 0.05) gain to feed than CON. The CON significantly higher (p < 0.05) water intake than other treatments on d 0 to 14. The MCZ 1 significantly decreased (p < 0.05) relative (ratio of absolute organ weight to BW) organ weights, including retroperitoneal white adipose tissue (RWAT) weight and inguinal white adipose tissue (IWAT) weight, compared to CON. In conclusion, our study suggests that there was no significant difference in the antiobesity effects between MC and CZ, and MCZ 1 has synergistic effects by regulating adipose tissue.


Anti-obesity, C57BL/6N mice, Chrysanthemum zawadskii var. latilobum, Momordica charantia, Water intake


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This research was supported by “Regional Innovation Strategy (RIS)” through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE) (2021RIS-001).

Conflict of Interests

No potential conflict of interest relevant to this article was reported.