Korean Journal of Agricultural Science (Korean J. Agric. Sci.; KJOAS)
Indexed in KCI (Korea Citation Index), Open Access, Peer Reviewed.
pISSN 2466-2402
eISSN 2466-2410

Analysis of essential oils by GC/MS and tilianin by HPLC/UV of the aerial parts of Agastache rugosa

CONTENTS

PLANT&FOREST

Tran GH, Choi J, Lee HD, Lee Y, Hwang JS, Kim DY, Lee HM, Lee S. Analysis of essential oils by GC/MS and tilianin by HPLC/UV of the aerial parts of Agastache rugosa. Korean Journal of Agricultural Science 50:63-73.

Korean Journal of Agricultural Science (Korean J. Agric. Sci.) 2023 March, Volume 50, Issue 1, pages 63-73. https://doi.org/10.7744/kjoas. 20230003

Received on 22 November 2022, Revised on 22 November 2022, Accepted on 06 January 2023, Published on 30 March 2023.

Analysis of essential oils by GC/MS and tilianin by HPLC/UV of the aerial parts of Agastache rugosa

Gia Han Tran1, Jungwon Choi1, Hak-Dong Lee1,2, Yunji Lee3, Jae Seong Hwang4, Do Yoon Kim4, Hwan Myung Lee4, Sanghyun Lee1,2,5,*

1Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea

2Natural Product Institute of Science and Technology, Anseong 17546, Korea

3Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Eumseong 27709, Korea

4Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan 31499, Korea

5BET Research Institute, Chung-Ang University, Anseong 17546, Korea

*Corresponding author: slee@cau.ac.kr

Abstract

Agastache rugosa is an edible plant belonging to the Lamiaceae family and has different names including Korean Mint, purple giant hyssop, Indiana mint, and wrinkled giant hyssop. A. rugosa is renowned as a vegetable and herbal medicine in conventional therapy. Many compounds of A. rugosa were detected by instruments for industrial use. To examine various compounds in A. rugosa, gas chromatography-mass spectrometry (GC/MS) and high-performance liquid chromatography coupled with an ultraviolet-visible method (HPLC/UV) was developed. Essential oils including those used to treat diseases were extracted by a steam distillation method and characterized using the GC/MS method. Tilianin, a polyphenol compound possessing antioxidant capacity, was then analyzed by the HPLC/UV method. The six compounds of A. rugosa essential oil were identified as 1-heptanol, n,n-dimethylvinylamine, limonene, isomenthone, 4-allylanisole, and pulegone. Among these, the most abundant component was 4-allylanisole, accounting for 90.4% of the compounds. The tilianin content from aerial parts of A. rugosa was 9.58 mg·g-1 extract. Tilianin thus may serve as a marker compound for evaluating the bioactivity and determining the therapeutic efficacy of this plant. A. rugosa containing these bioactive compounds is expected to be a beneficial resource for expanding industrial applications in the future.

Keywords

Agastache rugosa, essential oil, quantitative analysis, steam distillation, tilianin

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Authors Information

Gia Han Tran, Department of Plant Science and Technology, Chung-Ang University, Master student

Jungwon Choi, Department of Plant Science and Technology, Chung-Ang University, Master

Hak-Dong Lee, Department of Plant Science and Technology, Chung-Ang University, Ph.D. student

Yunji Lee, Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Researcher

Jae Seong Hwang, Division of Cosmetic and Biotechnology, Hoseo University, Doctor of Philosophy

Do Yoon Kim, Division of Cosmetic and Biotechnology, Hoseo University, Doctor of Philosophy

Hwan Myung Lee, Division of Cosmetic and Biotechnology, Hoseo University, Professor

Sanghyun Lee, https://orcid.org/0000-0002-0395-207X

Authors’ Contributions

G. H. Tran, J. Choi, H.-D. Lee: HPLC analysis, Y. Lee: sampling and experimental design, J. S. Hwang, D. Y. Kim: steam distillation, H. M. Lee: data analysis and data curation, and S. Lee: experimental design and writing.

Acknowledgments

This research was conducted with a grant from the Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Eumseong 27709, Republic of Korea.

Conflicts of interest

No potential conflict of interest relevant to this article was reported.