PLANT&FOREST

Analysis of essential oils by GC/MS and tilianin by HPLC/UV of the aerial parts of Agastache rugosa

Gia Han Tran1   Jungwon  Choi1   Hak-Dong  Lee1,2   Yunji  Lee3   Jae Seong  Hwang4   Do Yoon  Kim4   Hwan Myung  Lee4   Sanghyun  Lee1,2,5,*   

1Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea
2Natural Product Institute of Science and Technology, Anseong 17546, Korea
3Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Eumseong 27709, Korea
4Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan 31499, Korea
5BET Research Institute, Chung-Ang University, Anseong 17546, Korea

Abstract

Agastache rugosa is an edible plant belonging to the Lamiaceae family and has different names including Korean Mint, purple giant hyssop, Indiana mint, and wrinkled giant hyssop. A. rugosa is renowned as a vegetable and herbal medicine in conventional therapy. Many compounds of A. rugosa were detected by instruments for industrial use. To examine various compounds in A. rugosa, gas chromatography-mass spectrometry (GC/MS) and high-performance liquid chromatography coupled with an ultraviolet-visible method (HPLC/UV) was developed. Essential oils including those used to treat diseases were extracted by a steam distillation method and characterized using the GC/MS method. Tilianin, a polyphenol compound possessing antioxidant capacity, was then analyzed by the HPLC/UV method. The six compounds of A. rugosa essential oil were identified as 1-heptanol, n,n-dimethylvinylamine, limonene, isomenthone, 4-allylanisole, and pulegone. Among these, the most abundant component was 4-allylanisole, accounting for 90.4% of the compounds. The tilianin content from aerial parts of A. rugosa was 9.58 mg·g-1 extract. Tilianin thus may serve as a marker compound for evaluating the bioactivity and determining the therapeutic efficacy of this plant. A. rugosa containing these bioactive compounds is expected to be a beneficial resource for expanding industrial applications in the future.

Figures & Tables

Extraction procedure of collection of (A), extraction mantle of (B), steam distillation (C), and essential oil and hydrosol (D).