PLANT&FOREST

Effect of insect-resistant genetically engineered (Bt-T) rice and conventional cultivars on the brown planthopper (Nilaparvata lugens Stål)

Sung-Dug  Oh1   Eun Ji  Bae1   Kijong  Lee1   Soo-Yun  Park1   Soo-Yun  Park1   Myung-Ho  Lim1   Doh-Won  Yun1   Seong-Kon  Lee1   Gang-Seob  Lee1   Soon Ki  Park2   Jae Kwang  Kim3   Sang Jae  Suh2,*   

1Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea
2School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
3Division of Life Sciences, Incheon National University, Incheon 22012, Korea

Abstract

Insect-resistant transgenic rice (Bt-T) expresses a toxic protein (mcry1Ac1) derived from the soil bacterium Bacillus thuringiensis found in the rice cultivar Dongjin with an insecticidal property against rice leaf roller (Cnaphalocrocis medinalis). In this study, to investigate the impact of Bt-T on non-target organisms, the feed and oviposition preferences and biological parameters of brown planthopper (Nilaparvata lugens Stål) were comparatively analyzed in four rice cultivars: Dongjin (parent variety), Ilmi (reference cultivar), Chinnong (brown planthopper resistant cultivar) and Bt-T. In the Bt-T and Dongjin cultivars, the feed preferences were 32.4 ± 8.3 and 34.1 ± 6.8%, and the oviposition preferences were 32.5 ± 5.1 and 30.0 ± 5.3% respectively, and there was no statistical significance between these rices. Additionally, in the Bt-T and Dongjin cultivars, the total lifespans from egg to adult were 39.5 ± 6.9 and 40.0 ± 5.8 days, and the weights of adult females were 1.78 ± 0.14 and 1.72 ± 0.16 mg, respectively. Therefore, there was no statistical difference in the biological parameters between these two varieties. Overall, the results indicate that the insect-resistant transgenic rice (Bt-T) did not negatively affect the reproduction and life cycle of brown planthopper, a non-target organism.

Figures & Tables

Fig. 1. Feeding and oviposition preference test of brown planthopper (Nilaparvata lugens Stål) to tested rice varieties: Dongjin (parent variety), Ilmi (reference cultivar), Bt-T (insect-resistant genetically engineered rice), and Chinnong (brown planthopper [BPH] resistant cultivar). (A) 24 hours after infestation. (B) 48 hours after infestation. (C) 72 hours after infestation. (D) BPH feeding on rice. (E) Eggs of BPH. Red arrow: BPH.