Evaluation of the wound healing ability of an Abeliophyllum distichum Nakai extract in ICR mouse and of antibacterial activity against human cutaneous flora

Young Bin Go1   Ji Hwan Lee1   Han Jin Oh1   Yong Ju Kim1   Jae Woo An1   Se Yeon Chang1   Dong Cheol Song1   Hyun Ah Cho1   Jin Ho Cho1,*   

1Division of Food and Animal Science, Chungbuk National University, Cheongju 28644, Korea


In this study, we evaluated the wound healing rate and, inflammatory cells effects of by Abeliophyllum distichum Nakai (ADN) extract in mice. We also assessed the stability of the ADN extract upon exposure to sunlight. Treatments were as follows: 1) CON (only saline solution), T1 (CON + 0.0125% ADN extract), T2 (CON + 0.05% ADN extract), and T3 (CON + 0.5% ADN extract). A 4 mm punch was used in the central part of the dorsal area to separate it from the subcutaneous tissue, causing a full-thickness skin wound. An amount of 1 mL of each sample was sprayed onto the treatment section of the wound with a pipette every day from the day of wound creation, with proper application ensured using brush. In the stability test, the pH was measured at 1, 4, and 8 weeks after exposing the samples of each treatment section to sunlight considering, the higher concentrations of the ADN extract. The results of this study indicate that the effectiveness of the wound contraction rate in the mice to which the ADN extract was applied was low. Moreover, the stability of the sample containing a high concentration of the ADN extract could not be verified. In addition, no significant results were obtained in the inflammatory reaction assessment. Therefore, additional research focusing on wound contraction, stability, and inflammatory cell outcomes of the ADN extract is needed.

Figures & Tables

Fig. 1. Antimicrobial activites of Abeliophyloum distichum Nakai (ADN) extract against two strains ( , and ). The inoculum (about 1 × 10) was spread on the different media then, were grown at 37℃ at for 24 h ( ) and 72 h (). There was no clear zone against the test microorganisms. T1, CON + 0.0125% ADN extract; T2, CON + 0.05% ADN extract; T3, CON + 0.5% ADN extract.