ANIMAL

Antibacterial and anti-obesity effects of Abeliophyllum distichum Nakai: an in vitro study

Dong Cheol Song1   Ji Hwan Lee1   Han Jin Oh1   Yong Ju Kim1   Jae Woo An1   Se Yeon Chang1   Young Bin Go1   Hyun Ah Cho1   Jin Ho Cho1,*   

1Division of Food and Animal Science, Chungbuk National University, Cheongju 28644, Korea

Abstract

Interest in research on various medicinal plants has increased globally over the last few decades, possibly due to their possible antibacterial and antioxidant activities. The present study was conducted to verify the antioxidant effects, antibacterial activity, and collagen synthesis and cell viability outcomes of adipocytes upon exposure to Abeliophyllum distichum Nakai (AdN). Antibacterial activity was measured through the Disc diffusion method to compare the growth ability of pathogenic microorganisms (E.coli, Salmonella). The absorbance was measured at 560 nm to calculate the active oxygen scavenging ability. Fibroblasts were dispensed in a 96-well plate at a density of 1 × 105 cells·well-1. The amount of procollagen was measured in each case using a procollagen type 1 C-peptide EIA KIT. The cytotoxicity of the Abeliophyllum distichum Nakai extract against animal adipocytes (Hanwoo backfat cells) was determined using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, a method that measures the conversion of MTS to Formazan by means of mitochondrial dehydrogenases. The concentrations of the samples were made to be 0.0125, 0025, 0.05, 0.1, and 0.5% and all were -completely absorbed into the disc in an incubator at 37℃ for 24 to 36 hours. For the 0.125 mg·disc-1, effects of Abeliophyllum distichum Nakai on the antioxidant effect, antibacterial activity, and cell viability of adipocytes were found. However, Abeliophyllum distichum Nakai had no effect on collagen synthesis, thus suggesting that AdN extracts may be useful for the prevention and/or treatment of obesity.

Figures & Tables

Fig. 1. Antimicrobial activities of Nakai extract against two strains. The inoculum (about 1 × 10) was spread on the different media then, were grown at 37℃ for 24 h (E.coli) and 48 h (Salmonella). The antimicrobial activity was evaluated by measuring the zone (mm) of clear zone against the test microorganisms. CON, no additive; T1, 0.0125 mg·disc ; T2, 0.25 mg·disc; T3, 0.50 mg·disc; T4, 1.0 mg·disc; T5, 5.0 mg·disc.