PLANT&FOREST

Evaluation of the acute toxicity of Theoredoxin (TRX) transgenic soybean to Daphnia magna

Sung-Dug Oh1,*   Seok-Ki Min2   Jae Kwang  Kim3   Jung-Ho  Park4   Chang-Gi Kim4   Soo Yun Park1   

1Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea
2Korea Testing & Research Institute, Hwasun 58141, Korea
3Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Korea
4Bio-evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Korea

Abstract

Theoredoxin (TRX) transgenic soybeans were developed using the human Theoredoxin gene under the control of the β-conglycinin promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. This study was done to assess the acute toxicity of a genetically modified (GM) soybean using the fresh water planktonic crustacean Daphnia magna. The acute toxicity effect of the TRX soybean and non-GM soybean (Gwangan) on D. magna was investigated at different concentrations (0, 156, 313, 625, 1,250, 2,500, and 5,000 mg·L-1). The TRX soybean used for the test was confirmed to express the TRX/PAT genes by PCR and enzyme-linked immunosorbent assay (ELISA). D. magna feeding tests showed no significant differences in the cumulative immobility or an abnormal response with either the TRX soybean or non-GM soybean. The feeding study showed a similar abnormal response and cumulative immobility of the D. magna between the TRX soybean and Gwangan treatments. Additionally, the 48 h-EC50 values for the TRX and Gwangan soybeans were 755.6 and 778 mg·L-1, respectively. The soybean NOEC (no observed effect concentration) value for D. magna was suggested to be 156 mg·L-1. These results suggest that there is no significant difference in toxicity to Daphnia magna between the TRX soybean and its non-GM counterpart.

Figures & Tables

Fig. 1. Confirmation of the T-DNA genes on the (TRX) transgenic soybean and non-genetically modified soybean (Gwangan). M, 100 bp plus DNA ladder; N, non-genetically modified soybean (Gwangan); TRX, TRX transgenic soybean; , gene; , gene.